Scanned negative file is not recognized as photo file

SOURCE: Epson perfection 4990 photo scanner

Please do the following to scan slides or negatives in manual mode of the Epson TWAIN:
For scanning transparencies in HOME mode - for EPSON SCAN models, not Epson Twain/Manual mode models.
1) In the program you are working from, begin the scanning process. If working in FULL AUTO MODE as the scan begins click PAUSE or CANCEL, then change "Full Auto Mode" to "Home Mode".
2) You will now see all Epson Scan settings displayed. If the scanner has not previewed automatically, click the large preview button to get a visual of what is on the scanner.
3) Ensure the settings on the Epson Scan are correct for what you wish to scan. For example, the DOCUMENT TYPE for transparencies must be either "Color negative film", "B&W Negative Film" or "Positive film"*.
* FILM here indicates that the item being scanned is transparent to light; this setting is also used for slide scanning. Positive indicates that the image is in true colors (ie the grass is green and sky is blue), whereas negative indicates reversed colors, as produced in some photo finishing processes.)
If the DOCUMENT SOURCE is set for a flatbed related option, ie PHOTO, the scanner is looking for reflective items and will not activate the backlight in the transparency unit. If the DOCUMENT TYPES for "FILM" are greyed out or cannot be selected, close the Epson Scan, reseat the power/communication cable connecting the base of the scanner to the transparency unit, then reopen Epson Scan and try again.
NOTE for PROFESSIONAL MODE users: DOCUMENT TYPE should read TRANSPARENCY for film scans.
4) Ensure the other scanner settings are correct for the type of scan you are doing. For example:
IMAGE TYPE should be color photo for all positive or negative color transparencies, or BLACK AND WHITE PHOTO for monochrome transparencies.
DESTINATION is unimportant, however you should select a RESOLUTION appropriate to what you plan to do with the scans. (There are no hard and fast rules for DPI. As a general guideline, if you are saving scans at original size, a resolution ranging from 400-600 DPI should capture enough information to faithfully record the image. If the slide or negative will be enlarged, you will need to increase this DPI accordingly. We have no means to calculate what your exact needs will be in this situation; you will need to experiment and view the output to gauge the appropriate input DPI from the scanner.)
PREVIEW. If your scanner is doing a THUMBNAIL PREVIEW, the slides will automatically preview and appear in boxes on the left side. Here, you can select or deselect the slides you wish to scan.
If you recieve a message that the scanner could not find the film, or you see in the preview that part of the scan is cut off, click the down arrow next to the large PREVIEW button and click NORMAL, then PREVIEW again. In NORMAL PREVIEW mode, the scanner will capture all information on the flatbed; you will then have to manually marquee or crop the slides you wish to scan. Use the left mouse button (click, hold, drag) to lasso one slide inside a box made of dotted lines, then click scan. Only the selected area will scan. Repeat this process for as many slides or filmstrips are on the bed.
5) Click CLOSE once scanning is complete. The images will now transfer to the application in use.

SOURCE: I cannot get clear scans from my photo negatives

If you are seeing pixelation, the issue is either insufficient resolution during the scanning process or a problem with the output device. If it's a focus issue, which will NOT cause pixelation, the problem may be the height of the negative holder. The Epson Perfection V series scanners have a fixed focus point which only adjusts between two settings depending on whether you select the film holders (which raise the film off the glass) or the film guide (which scans film directly on the glass) in the software options. If you are going to scan transparencies or negatives, familiarize yourself with the holder height adjustment issue and be willing to experiment with different height settings until you are getting optimum results. This is not documented much at all in the Epson user manual but there is a little bit about it, including some examples of results at different settings, here: http://www.photo-i.co.uk/Reviews/interactive/Epson%20V700/page_13.htm Apparently every scanner is slightly different so even though the lowest holder foot setting (default) is working best for some, other settings work better for others. On my V700, I have the feet on the film holders set to the highest setting plus I shimmed them with a 1mm piece of styrene before I could get the scans in focus. Once I found the right height though, I've been able to get beautiful scans from this machine. BTW, scanning negs directly on the glass will almost always result in Newton Rings.

SOURCE: Epson Scan Reporting "Unable to write file" when scanning a photo on a Mac.

Do you have sufficient disc space? That is the only reason you should see an error like this.

SOURCE: Epson V300 Photo Scanner Streak When Scanning Negative

I also own a v300, and I have noticed that I occasionally get rainbow streaks if I bump the scanner at all while it is scanning negatives, so this might have something to do with vibrations in the machine. Is it sitting on a stable surface?

SOURCE: epson v500 not able to scan negatives! When I open

You probably need to reset (not reload) the scanner software. Start the Epson scan software. Select professional mode from the pull down menu (it lets you choose between auto, home and professional.) On the next screen select at the very bottom select configuration. The next screen should have 4 tabs, select other. The at the bottom (I think) select restore to factory defaults. This will reset your software, now you can use auto mode if you want it to make all your selections or home if you want some control or professional if you want to control everything. Don't forget to use your film holder that came with the unit and remove the white cover that is locked underneath the lid that covers the film illumination bulb.