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Re: Image rings when using Coolpix for microscopy
Under some circumstances when using the Coolpix 4500 attached to a microscope concentric rings are visible as in the below.
The COOLPIX 4500 aspherical lens surface is ground to create the ideal curves to gather rays at the focal point with an extremely high accuracy. Such minute lines are not visible in the image taken with a camera alone, however if the camera is used with a microscope, the lines become visible as the depth of field is increased due to the combined F numbers of the camera lens and microscope lenses.
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the methyl orange and methylene blue are stains for use on thin
plant sections to show cell structures.
the gum is to attach coverslips to slides.
Try Googling microscopy there is one particularly good site
something like microscopical discussions but you could start with
I did a bit of research, and it looks like the only way that you can use the "Mac OSX compatible" microscope, is using it the same way one would plug in a webcam via USB. Essentially, you can plug in the Microscope, open up the Image Capture Utility, PhotoBooth, or Quicktime/iMovie (I'd recommend one of these two), make sure you have the Capture Device set to the microscope and you'll be able to "project" the image that you are seeing through the microscope onto the computer.
The Motic software does not have a counterpart for the Apple OS, and can only be used if you have a Windows BootCamp Partition, or if you have a Parallels installation of Windows that can use the Motic Software. There are other pieces of software out there that can utilize microscopes, but may not have all the same features as the Motic Bundled software. One that I found that may be of some use is called miXscope. It can be found here: http://www.edhsw.com/mixscope/ , and it is relatively inexpensive in terms of licensing.
This may also be of some help for selecting compatible microscopes in the future: http://www.apple.com/science/solutions/microscopy.html
Hope that helps!
Seems to be a tiny microscopic chemical dot or damage on the lens. Or damage of the image sensor. Did you tried to capture the direct sunlight, or did you photographed the sun (few times totally enough). Surely burned the image sensor. Usually occurs when camera being used in bad whether conditions in out side for long time. In you case better consult a professional camera service personal.
If you are initiating the shutter manually, it is quite possible that the camera has become very slightly loose at the F-mount. So, when you press the shutter button, the movement though it may be extremely slight, is shaking the camera at the exact moment you are taking the picture.
Make sure that the camera is tight to the F mount and that the F mount is secure to the microscope.
If you are initiating exposure via software, it is still possible that there is still some slight movement on your desk.
Remember, the slightest movement is magnified many, many times to the camera.
if you check the operation for spliting to a photo tube. PG 49
you can see the procedure for using a camera
(there is a control pin on the side of the scope (shown on pg 49) which you have to operate to switch to the camera output... you should then be able to take your photos :)