Question about Tasco 750 Monocular Microscope

Problems with viewing under low magnification (20x) only red stain

I was looking at cells under low magnification (20X) and noticed that
if I tried to view my stain on these-- dapi, rhodamine; the dapi worked
fine,light seemed to be aligned correctly. However, the red stain did not
show. It appears the light path is blocked -with two circles meeting half
way cancelling each other. This only happens and is the most obvious under
low magnification and with the red stain. If I try to view this with 60X,
I am able to get some of the red, however, you also see a halo - a part of
the field of view with bright light coming through. This is just a recent
development as I had no such problem last week.

So, my question to you, what could be causing this and how can I fix
it????
Any help or advice would be greatly appreciated.

Thank you so much for you time.

2 Answers

Anonymous

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30 Answers

Wow!

Sounds like you are using a Phase Contrast Microscope to me, and if this is the case, you will get just this kind of result, especially if it is not set-up correctly.
Let me have the make and model, and I will happily talk you through this one.

Kind Regards......Dave

Posted on Mar 10, 2008

Anonymous

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7 Answers

Hello..

For the red colour partical your illumination angle should be 0 degree.
As per you exepirience you are getting red at low magnification and not getting at high magnification. at the higer magnification the light deviation you can apriciate more then lower.

try to adjust your light source focusing lenc at higher magnification so at the lower it will be more better,

for the further problem you can contect me any time,

regards.

INDRAVIJAY

Posted on Feb 20, 2008

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