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The first thing I'd try is to run the scanner with the Nikon software. When you power on the scanner it goes through a variety of checks, and finally you should have a silent scanner with a solid green light at the front. Start the Nikon software and wait until it finds the scanner, if it doesn't you have a hardware problem with the scanner.
If it goes well to this point insert a card or plastic mounted slide and hit Preview. The scanner should then create and image you see on screen. Next step would be to make a scan. If the slide you're trying to scan isn't card or plastic mounted you'll need the Nikon made carrier.
I have many years reparing canon scanners so here is my suggestion to you.
When I do my scanning, and by the way I own a canon pixma also. I do it through the software I never hit the scan button. You can do it through Microsoft paint by using from scanner or camera and aquiring and image. I use paint shop pro so I just open that program and just aquire an image from my scanner. I hope this helps you out but I think its a software problem, because the software takes full control of the scanner if you do it the way I just suggested.