I have a 99 jetta 2.8 vr6 no spark on 2 and 5 plugs It just so happens that there is no signal from ecm to coil of 2 and 5 coil.

The ECM has detected random multiple cylinder misfiring (code p0300). Possible causes are spark plugs,high tension leads injectors ignition coils wiring and low compression As it is random suspect coils and wiring

Check to see if the cam position sensor or the crank position sensor is misaligned or has a corroded connector plug. The ECM needs to get a firing pulse from all cylinders. If the engine is a dual cam and is not attempting to start, some engines have a timing device (chain) for each bank. If the chain broke on one bank, the cam sensor can not possibly fire because the cam is not turning.

This is the firing order for the vehicle: 1-5-3-6-2-4.

pull the multi plug connectors off the coil pack and check the connections ,and then rub the pins with a soft lead pencil and see what happens

For the 2.8L DOHC V-6:
Firing Order:
1-5-3-6-2-4
Cylinders:
......(back).......
|.1....3....5....|
|....2....4....6.|
Ignition Coil Connections
(back)
|.2..5.|
|.4..3.|
|.6..1.|
(front)

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check your injector coil fuse under your hood

You must verify your work.

1. Test for spark. Make sure the coil is producing spark.
2. Did you remove the plug wires one at a time? If not then check to make sure the firing order is correct.

did you check compression cilinder?maybe you have the pocelanite spark plug craked or the rubber coil protection damage

You may have a bad coil pack - try moving two of them around and see if the misfire follows the coil pack or stays at the same cylinder. If it follows the coil pack, get a new one. If it stays in the same cylinder, inspect your spark plug wires and make sure the plug is gapped correctly.

1.1 mm is the standard gap for a platinum tipped plug. Plugs for this car usually come pre-set but it's always worth checking before installing. Hope this helps.