Science & Laboratory - Page 4 - Recent Questions, Troubleshooting & Support
Can I put a 40x microscope lens into an ultrasonic
Soak in a product like lime away first. Glass lenses cant warp without heat. Plastic even crystal type can absorb minerals. Ultrasonic can help clean all of them. The lime away will remove almost all trace minerals, lye or powdered drain cleaner will remove organics. Gool luck let me know how these ideas woek for you.
on Jan 05, 2018
I have inherited a Nikon
I think the number you gave is the microscope serial number, to hope to get help you need to find the model number so people can help. At least post a picture. In general the microscope needs a light source to be bright enough to use. The transformer is meant to provide power to the light. If you use the microscope with a light shining through the object held by a glass slide then the light is below the stage which holds the object. Sometimes on simpler microscopes they have a mirror below the stage and you shine a bright light beam into the mirror and must adjust the angle to reflect the light through the slide. In more complex systems the light is built into the microscope. For opaque objects you must shine the light from above onto the object. If you can't find the official Nikon light source to connect to the transformer, you may be able to get non Nikon light sources. For my stereo microscope for example, I use a LED ring-light I bought on ebay for ~$25. This lights the objects from above and fits around the microscope tube just above the stage.
on Dec 27, 2017
How will you track a moving image in the microscope?
Your question is hard to answer without more detail, including movement rate and required magnification. For relatively slow moving objects you can use a movable stage (X-Y stepper motors tied to a computer). Usually the direction of motion is erratic or unknown, and some digital tracking software will have to be written to drive the steppers so as to follow the designated object of interest. This may be something you have to develop yourself, although there may be some "tracking" software commercially available (or adaptable).
Note that you usually cannot take a video of the object as you track it, due to the quantum action of the stepper motors. However, this does not keep you from taking a series of stills (step - capture - step - capture - step ... etc.) and turning them back into a stop-action video. There are a lot of free or cheap programs out there to do that.
If the motion of the object is rapid, then you have a much bigger problem. One project I did some years back had this problem, and we could not actively track the sudden motion with a microscope. What we did instead was to reduce the microscope magnification, and significantly up the resolution of the high-speed camera (and yes, that was very expensive). At 1500 FPS, we could capture the object as it moved rapidly across the stage, then post-process the resulting frames to center on the object. The obvious limit here is that the object needs to cover a relatively small portion of the overall picture - which limits the magnification you can use. Of course, the offsetting advantage of this method is that you pay more for the camera, but don't have to have a movable stage on the scope.
on Dec 06, 2017
Vivitar 40 piece microscope set shrimp hatchery instructions
1. To hatch the eggs, first prepare a brine solution. Pour the entire contents of a vial containing sea salt into a quart of
tap water. Add the brine shrimp eggs into the
solution. Allow the solution to stand at room
temperature (70 - 80F or 21 - 26C) for 24
to 48 hours and the eggs will hatch into nauplius larvae (this is the first stage of development after leaving the eggs).
2. Place some of the larvae into one of the compartments of the shrimp hatchery .
3. Place some fresh brine solution in another
compartment. Add a small amount of yeast to
this new solution. Then, using the eyedropper, transfer some of the larvae into this compartment as well. The yeast will
serve as food and produce oxygen for the larvae as they develop into maturity. Without food and oxygen, the shrimp cannot develop
and will die. Mature brine shrimp are known
as Artemia Salina.
Note: Using an eyedropper with just the right
pressure to get a desired amount of liquid
onto a slide can be harder than it looks. Take
out a clean slide and practice squeezing a
drop of water onto the slide until you feel
comfortable that you can control the size of
the drop that you're squeezing out.
4. Observe the life cycle of the shrimp as they
grow: the dried eggs, the hatching eggs, the
developing larvae, and finally, the mature
5. The mature shrimp may be fed to fish in an
aquarium if you so wish. However, first
remove the shrimp from the brine solution
and place them into fresh water. An increase
in salt may harm the fish in the aquarium.
NOTE: Use the color filter especially when looking at clear or dim specimens.
Vivitar 40 Piece...
on Nov 27, 2017
Fischer Scientific AB15 -- Unable to calibrate or take any readings.
The electrode you have is a combination electrode with an integral calomel reference half-cell and built-in temperature probe. Since the temperature reading is good, the temperature probe is okay. It isn't part of the pH circuits anyway. (Your meter uses the temperature to calculate a temperature correction of pH.) Something else is going on with the electrode proper. Its funny behavior suggests to me that it has dried out internally.
pH electrodes don't last forever. How old is this one? If it's an old one, it's probably time to replace it. If it's new and doesn't work, it may be under warranty.
If you shop for a new electrode, get a gel-filled one. And go for one with a silver/silver chloride reference instead of a mercury one -- especially if you are using it on food or other samples that may be consumed. Another consideration about calomel electrodes: they take hours to recover from relatively small temperature changes. Ag/AgCl references aren't as sluggish.
Finally, take care when storing the electrode. It should be stored under humid conditions to keep it from drying out. The best way is to put a small piece of cotton or glass wool soaked with distilled or deionized water inside the plastic end cap. For shorter-term storage, e.g., overnight, you could immerse the tip in pH 7 buffer.
Fisher Science &...
on Nov 22, 2017
Why does my mercruiser 4.3 sputter and backfire after hot
It sounds like a fuel vapor lock, check that the fuel line to the carb is not coming contact with any metal surfaces of the engine for this will boil the fuel in the line before it enters the carb. If this is the case, just stand the fuel line off from where it is touching. I hope this helps....GOD BLESS....Randy...
on Nov 07, 2017
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