Since there's only a very small amount of light reaching the lens system of microscopes when on high powers, A special technique (called >>>oil immersion
Not using this technique must be the cause of the problem you are experiencing.
eHow will guide you through the exact procedure of using the oil immersion technique: http://www.ehow.com/how_8431358_focus-high-power-objective-microscope.html
Good luck in exploring the micro world!
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