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Anonymous Posted on Jul 09, 2011

Microscope will not focus on high power cloudy

1 Answer

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  • Expert 96 Answers
  • Posted on Jul 09, 2011
Anonymous
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Joined: Mar 04, 2011
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Since there's only a very small amount of light reaching the lens system of microscopes when on high powers, A special technique (called >>>oil immersion
Not using this technique must be the cause of the problem you are experiencing.
eHow will guide you through the exact procedure of using the oil immersion technique: http://www.ehow.com/how_8431358_focus-high-power-objective-microscope.html
Good luck in exploring the micro world!

  • Anonymous Jul 09, 2011

    I apologize for part of the answer missing, it should be as follows: "...(called >>>oil immersion<<<) is required to observe specimens with sufficient clarity". However, this has no effect on the answer itself.

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Related Questions:

1helpful
2answers

What is the other types of microscope ? how do they differ from a compound microscope ?

Compound Microscope
  • Compound microscopes can be found in most biology and science classrooms. They are electrically operated and use light to enhance the image of a cell. They will have multiple lenses for viewing.
Dissecting Microscope
  • Dissecting microscopes are also known as stereo microscopes. They have low magnification and are also light powered. These microscopes can view objects larger than what a compound microscope is able to handle, in three dimensions.
SEM
  • A Scanning Electron Microscope uses electrons instead of light to create an image. These microscopes produce three-dimensional images with high resolution and magnification. They also have a larger depth of focus.
TEM
  • Transmission Electron Microscopes use electrons instead of light to create an image. The material prepared must be very thin. The beams of electrons that pass through it give the viewer high magnification and resolution. These give two-dimensional images.
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1answer

After focusing it drifts out of focus. The knobs do not stay in the same place

There is a tension adjustment just inside the coarse focus knob. (same side as the switch) It looks like a chrome ring with 2 or 3 holes in it. You may have an odd looking tool that came with your microscope that is used to make this adjustment. Tighten it just a bit until the stage stops falling. You don't want it too tight or it will begin to make it difficult to adjust your coarse focus.
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1answer

Why should the coarse focusing knob not be used when focusing with the higher-powered objective lenses

There are two reasons. One reason is that when on high power you are working so close to the glass slide that it is easy to misjudge how much you are moving the specimen toward the lens that you can break a slide before you realize it. The coarse focus moves the stage with the specimen on it very fast and you only have a very very short distance within the focus plane before you run the objective lens into the slide.
Secondly, it is just harder to control the minute adjustments needed at the higher powers with the "coarse" focus knob. If you start at the low magnifications find what you want to concentrate on with the coarse focus knobs and then work your way up to the higher powers, you will have very little trouble moving to the fine focus controls at 40x and 100x while still having control of your image.
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1answer

My premiere microscope MS-01 Ultra will not focus

There is what is called a "stage stop" screw. Its purpose is to limit the specimen from rising high enough to contact the objective lens. The "stage stop" screw is directly behind the black stage platform in plain sight and very easy to access. It is a thumb screw and sits vertically. Simply loosen it a bit to allow the specimen to rise high enough to focus.
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1answer

My microscope won't focus, i tried everything...i don't know what is wrong with it...it's brand new.. it worked a couple times but now it just stopped.. i dont know what brand it is...all it says is...

If your stage is still in its correct orientation then make sure your objective lenses are screwed in tight as well as the eye piece being secured correctly. Try using different objectives (don't go too high or you may damage your lenses if you ram it into your specimen) and using the fine and course focus. If the you still cant focus using different objectives it's quite possible that your mirrors or lenses inside the microscope have been knocked loose. I would then recommend only having it repaired by a certified microscope technician. If it is still under warranty take it back to the store of purchase or send it back to the manufacturer.
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1answer

Stage slowly falls causing unfocus

Try holding the Right knob so it does not move, whilst firmly moving the Left knob towards you (clockwise), this is the adjusting method used on Zeiss Jena 1960's High End microscopes, e.g Eduaval & Ergaval.
Good luck,
Alan Mowle
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1answer

Nikon Optiphot stage is falling

This might be sign of trouble, because the Nylon gears inside might be worn out. I did not noticed a tension ring on Optiphot 1 or 2. The Nikon S and Metaphot from 1960s to 1976 have Nylon gears with tension ring adjustments.
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2answers

Microscopes

sir,
Diffrent company having their own system for microscope arm balacing.

Tell me which one microscope you have.

Auxillary arm or microscpe body sloly come down ward.

tell me problem is consult with which part..

INDRAVIJAY
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Microscopy; Relatively large spots on unknown surface obstructing view

HELLO..

There is five part in microscope

1. Eye piece

2. Binocular tube

3.Objective

4. magnification changer or joom accembaley

5. illumination segment

Try to make it out which part is gives a effect to your vision.

as per my suggesition illumination asembely you just clean it first.

keep in touch

regards

INDRAVIJAY
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High Power Objective

the objective is dirty with oil. Clean this off and it should solve the problem. The oil if it has been there for a long time may dry and become hard to remove.
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