Hi Ulrike
I suspect that your backup software has "locked" the drive / library from the front panel.
This is why disconnecting / rebooting the library solves the issue.
If you are using backup exec or similar, try going to devices, selecting the library, right click and "unlock" before using the front panel buttons.
You could also use BUEX to do the tape moves for you instead of even touching the front panel.
If you are in the UK and are still having problems then click the link below and we will do what we can to help.
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