Question about Motic DMB5 Binocular Microscope

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Our Olympus BX51 light source is bright in the center of our spcimen and then darkens as the field moves out of the center. We can not get the same amount of light to cover our whole specimen.

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Under all objectives or only low power? is the condenser centered ? if yes try a diffusion filter

Posted on Jul 16, 2008

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How can I fix my screen ,will not show anything on the field analyzer 740i? Help please


Did you move the brighness control. It is on the right side of the equipment. If not, it could be the video board inside of the equipment

Aug 05, 2014 | Zeiss Humphrey 740 Visual Field Analyzer

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I'm using the Nikon ECLIPSE e800 microscope and for some reason there is a shadow on half of my slide. Also, I can usually find and take pictures of slides with the lamp adjusted at about 5 or so but...


There are a few things that could cause a shadow in the field of view. First if the condenser is not centered, this can create a crescent shaped shadow and rob your image of light. Center the condenser and raise it to within about a 16th of an inch from the top of the stage.
Second, the slider on the head which diverts light from the eyepieces to the camera must be fully engaged to the "camera" position
Third, make sure that the diaphragm on the condenser is not closed too far. There is an adjustment ring for this. Set it to 70-80% of the numerical aperture of the objective you are using.
Fourth: if there is a "swing out" condenser lens you will need it completely in or out depending on the objective you are using. for 2x-5x you need the swing out condenser lens completely out of the light path. For objectives 10x and up you need the swing out condenser lens completely in and centered in the light path.
If these things are properly adjusted you should not have a shadow in the field of view. If you still have a problem, let me know.

Dec 17, 2010 | Nikon Microscopes

1 Answer

How to troubleshoot a microscope having uneven brightness in the observation field?


uneveness of of brightness in the optical field of view (FOV) can be caused by a number of variables, but often times is do to miss alignment of the condenser. Typically when this is the problem there the "hot" (brightess area) will be shifted to one corner or another in the FOV. There are usually some sort of adjustment screw underneath the stage that will allow you to do the alignment. It is easiest if you close down the iris on condenser and adjust the height of the condenser until you can see an octagonal shape appear, then center it in the FOV.

If the uneveness is equally the same throughout the FOV then it may have to do with the position or the height of the condenser. Generally speaking the lower the magnification, the further the condenser should be away from the slide. The condenser narrows or broadens the light allowing you to fill the entire FOV of the objective.

Depending on the quality and the level of the microscope though, you may find that no matter what you do at lower magnifications you can do very little to improve this. I see this a lot with LED models. The dispersion pattern of an LED is much more focused then a halogen, which is GREAT for higher magnification, but often times manufactures will upgrade from halogen to LED an never change the optics of th collector lens and condenser to flatten out the illumination better.

Nov 19, 2010 | Olympus Microscopes

1 Answer

Uneven brightness in the observation field


Most likely your objectives are misalingned. Tighten the objective wheel, that usually solves it.

Nov 10, 2009 | Olympus Microscopes

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Parfocal microscope


Are you able to focus on the specimen? Is the problem the same if you switch to a higher magnification lens?
Could be several things, a slider half way in, a turret not clicked in to the notch, ie condenser, a filter partway in.

Jan 27, 2009 | Microscopes

2 Answers

Problems with viewing under low magnification (20x) only red stain


hello..

For the red colour partical your illumination angle should be 0 degree.
As per you exepirience you are getting red at low magnification and not getting at high magnification. at the higer magnification the light deviation you can apriciate more then lower.

try to adjust your light source focusing lenc at higher magnification so at the lower it will be more better,

for the further problem you can contect me any time,

regards.

INDRAVIJAY

Oct 11, 2007 | Tasco 750 Monocular Microscope

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