Wireless mice have two parts. The mouse which needs regular battery charging and the receiver. The receiver is a USB device that plugs into any of the USB ports on the back (near the DVI port) or on the side below the audio jacks. Plugging into a USB hub is an option but can give an extra area of trouble-shooting when it fails.
If the port doesn't work, try a different one. Check that the USB ports aren't disabled for security or power-savings. Make sure the receiver is showing that it is active and that the mouse has good batteries.
One of the biggest issues can be waking from sleep with a USB device. This is changed in the advanced power savings in Windows.
I hope this helps.
Cindy Wells
(since my wired mice need to be replaced every few years, I suspect wireless mice have similar failure issues.)
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