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There is a possibility that the flash drive might have failed. Try using the Flash drive on a different computer and if the same works then you may have to download drivers as suggested by Steve Butterworth.
I suggest obtaining a WIn7 or WIn8 boot up version on a USB drive so you can determine if the computer is even bootable. If that is not practical then you will need to provide some details such as OS version, hardware errors, what you changed at the CMD prompt, etc.
look for windows vista minimum requirments here >>
Windows Vista Home Basic
Processor 800-megahertz (MHz) 32-bit (x86) processor or 800-MHz 64-bit (x64)
System memory 512 MB - bytes (MB).
Note system configurations that use system memory graphics memory, at least 448 MB of system memory must be time operating system after some memory is allocated to graphics.
Card DirectX 9 class graphics
Graphics memory 32 MB - bytes (MB).
Hard drive size 20 Giga - (GB) in which the free space 15 GHz - (GB).
DVD drive internal or external
Internet access capability
Audio output capability
If two computers(and two different operating systems) arenot detecting the pen drive then most likely there is something wrong with thatpen drive and it needs replaced.
In Windows 7 you can check the following. Plug the pen drive into an available USB port. Click the Windows Orb(Start menu in XP).
Right-click on "Computer"(My Computer in XP) thenleft-click "Manage". If "Computer" does not exist in the "Start" menuthen it should be on the desktop. In the left pane click "Disk Management". In the right pane, the first column will show you the drives currentlyinstalled(C:, D:, E:, etc). C: is typically the drive your operating system isinstalled on. To check what drive is what, just click the Windows Orb(Startmenu in XP) then click "Computer"(My Computer in XP). If you see the same driveletters under "Computer" that you see in Disk Management then the pen drive isnot being detected.
Do you hear any sounds coming from Windows when you plug inthe pen drive?
If you have a different pen drive is that one beingdetected?
Are other USB devices detected when plugged into thecomputer?
Please check if the Computer supports SD card of larger capacity. If preinstalled OS is Windows XP Operating System, supports SD card from 512 MB to 2 GB max and does not support SDHC card. Windows Vista and Windows 7 Computer supports SD and SDHC card upto 8 GB to 16 GB,.