Question about Canon CanoScan LiDE 25 Flatbed Scanner

1 Answer

Hi there, I have a Canon Flatbed scanner (Lide 25) and when i try to scan i get an error message as follows: 'Unable to load TWAIN server'. How can i fix this?

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  • 6 more comments 
  • jasonterry May 14, 2009

    Hi Alstech,

    I have uninstalled, re-installed many times before. however, i have just tried again with no success. Any more ideas?


  • jasonterry May 19, 2009

    Hi Al,

    I have downloaded the uninstall software. Run it then reloaded the scanner drivers etc from the original CD and i still get the same error message. ie 'Unable to load TWAIN server'. Any more ideas?

    Thanks, Jason

  • jasonterry May 19, 2009

    Hi there Al,

    I'm running XP Home Edt, Version 2002, Service Pack 3.



  • Anonymous May 20, 2009

    scanner shows in control panel, but does not work with any photo imaging programs (windows doc imaging, etc)

  • jasonterry May 21, 2009

    Hi Al,

    Thanks for the continuing support. I have just checked for the files and the only one i can find is the Twain.dll file. The other 3 are nowhere to be found! Any ideas how i can get them back?



  • jasonterry May 21, 2009

    Just to confirm - i have SP3 installed on my PC!

  • jasonterry May 22, 2009

    Hi Al,

    I have sent you an e mail from my e mail address.



  • jasonterry Jun 07, 2009

    Hi Al,

    Thanks for your help - re-installing the twain files worked - I'm now scanning away freely again!

    Thank you so much.



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  • 105 Answers

Hi Jason

A Simple fix just reinstall your software that came with the scanner and this should re establish the link between twain and your scanner

Regards Alan (alstech)

Please dont for get to leave feedback if this was helfull

Posted on May 13, 2009

  • 2 more comments 
  • Alan Rothery May 14, 2009

    Hi jason

    further investigation reveals that the drivers dontuninstall correctley when you use the windows uninstall options

    try the link below download and uninstall your drivers using the canon uninstal then reinstall hopfully that should cure it

    Regards Al

  • Alan Rothery May 19, 2009

    Hi jason

    can you confirm which version of windows youare running and i will script a reinstall if twain

    regards Al

  • Alan Rothery May 20, 2009

    Hi jason

    Here we go then first of all check that the twain associated files are present and correct by using windows search and checking for each one in thw windows directory

    The four files of interest are in the /Windows folder.

    if they are all present try to re register the two dll's as services to ensure that they are running correctly

    click start Run then type regsvr32 twain_32.dll then click ok

    click start Run then type regsvr32 twain.dll then click ok

    restart pc the hope and pray ! .

    if no good

    The only other thing i can think of is to uninstall sp2 and your canon software reinstall sp2 and then the software

    people have reported various imaging problems after installing sp2 (information from MS technet )

    Regards AL (Alstech)

  • Alan Rothery May 21, 2009

    mail me on and i will forward them to you

    Regards Al



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Related Questions:

1 Answer

When i try to scan i get this message: unable to open TWAIN source Please check connection then re-start Toolbox the connections are tight

Driver mismatch - the driver of the scanner has forgotten the location of the TWAIN driver because another software has been using it lately. Follow this

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Error message

Hi toolpusher

Try going to CanoScan Toolbox > Settings and reset the 'defaults' button at bottom... this will restore factory settings and hopefully fix your problem... The other error message you can expect to get is "unable to scan - insufficent memory, check your **** space' (or something similar)... same fix works for both! ...Have had both messages on mine, sounds like a semi-regular conflict on some sort, but seems to be easily fixed. Good luck :-)

Sep 01, 2008 | Canon CanoScan LiDE 70 Flatbed Scanner

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TWAIN error

Connect the scanner and re-install the driver. That should fix it

Jul 10, 2008 | Canon CanoScan LiDE 25 Flatbed Scanner

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Unable to select TWAIN source

tried uninstallin and installin canon scanner drivers..nothing works..still seeing unable to select twian source..

so i tried an we go..
on vista 64 os/vista any Start>All programs>Windows fax and scan

next click new scan on top and that got me going.

some say there are issues with the way usb talks to old scanners with new operating systems like mine (vista 64). hope it helps until you find the actual cure.

Jun 24, 2008 | Canon LIDE20 Flatbed Scanner

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Your post for CanoScan LiDE 25

Hi there, yes I did everything per your instructions, Toolbox is installed and it still says, "release the lock switch, detach I/F cable and reconnect. Scanner driver will be closed" and then after clicking on that it says "Unable to open TWAIN source. Please check connection then restart toolbox" I have been to the Canon site and downloaded driver sites and it says everything is working. but when I go to scan it comes up with the boxes I said before. Quite lost at the moment? Thanks Maureen. PS I have unstalled and reinstalled

May 22, 2008 | Canon CanoScan LiDE 25 Flatbed Scanner

2 Answers

Canon scanner

TWAIN is the type of scanner driver that is used for this scanner. It is probably the most common type of scanning driver.

Did you install Toolbox? Not sure? Assuming this is Windows, click Start, All Programs, Canon. Look for open CanoScan Toolbox (4.9 I think). Once this is open, click the 'Settings' button. There should be a drop-down menu for 'Select Scanner'. Make sure this shows the CanoScan LiDE 25. If that scanner is not listed, then the scanner driver is not being seen or may not be installed.

Once it has been verified that the LiDE 25 is selected, click OK. Next, click the 'Save' button. Check the box for 'Display the Scanner Driver'. Now click the 'Scan' button at the bottom.
The next window that pops up should be ScanGear. If this happens, then everything is installed and the scanner is being detected properly. Attempt to scan or preview.

If there was a particular application you were trying to use and you are still having issues with it, please give those details.

May 19, 2008 | Canon CanoScan LiDE 25 Flatbed Scanner

1 Answer

Lock Switch and IF cable

Mine was doing the same thing. When I get on the program, before I click "acquire" to preview and scan, I click OFF the toolbox and then everything seems to be okay and I can go ahead and scan.

Nov 27, 2007 | Canon CanoScan LiDE 25 Flatbed Scanner

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