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Posted on Apr 13, 2009

Need to know what type of oil to use

I just inheirited an olympus clinical microscope with an oil lens. Does it matter what type of oil I use? the numerical aperature of the lens is 1.3.

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  • Posted on Apr 14, 2009
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Immersion Oil made for microscopes today is non fluorescing. You should be able to use any immersion oil on the market.


* Tip - After using your 100x oil objective, make sure to clean the lens thoroughly, as cross contamination to the 40x objective is a common occurrence. It will save you a lot of headaches down the road.

  • Anonymous Jul 01, 2009

    Nikon makes a non fluorescing immersion oil but it's not necessary for non fluorescent microscopy. You want to make sure it's PCB free.

    Nikon Part # MXA20234 $25.00 for 50cc
    Nikon Part # 77003 $12.00 30cc of non fluorescing type DF

    You can order it from any [b]Nikon Microscope[/b] supplier.


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Not using this technique must be the cause of the problem you are experiencing.
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