Question about National Optical 131 Monocular Microscope

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Can stained bacteria be seen with a 40x magnification microscope? not in detail, just enough to determine coloration of individual bacteria

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Hello--I understand that you need at least 600x to view bacteria and some say 1500+ so 40x doesn't seem to be in the right mag. area at all--not sure this helps much.You could purchase a 100x obj. and 10/15x eyepieces but will probably be into oil immersion for the obj.Possibly a 20x eyepiece + a 60x obj will give you a degree of definition.I use a SEBEN Bino with this combi and it gives a small view but not good.kind regards martin r

Posted on Mar 27, 2009

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Ya make sure u r not using too much oil,the lens r clean(use toluene for cleaning the oil)

Posted on Jun 26, 2009

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Mf 2 model - where do you put the cedar oil when using 100x magnifcation. i have read the manual and do not understand. i am a novice


The oil goes between the cover slip of the slide and the objective tip. It can also help to put some between the condenser lens and the bottom of the slide. Be careful when using oil, as when rotating to lower magnifications, it is very possible for the 40x to get oil on it. The 100x is sealed to protect it, but the 40x is not. If oil gets on the 40x, it can seep up inside and destroy the lens.

It is also a very good practice to clean both the 100x and 40x after each use. If the oil sits on even the sealed 100x objective for any long extent of time, it will start to break down the silicon seal, and you can potentially have the same problem as with the 40x, where the objective acts like a wick of a candle and sucks up the oil, thereby destroying the objective.

Jan 20, 2011 | Premiere Microscopes MF-02 Binocular...

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I have a Swift 3200 series, the 4D and 10D works 40D and 100D will not focus. any Ideas address is not my work address I will reply with business address if serious.


Since you need to use oil at 100x, there is probably a good chance that there is oil on both the 100x and 40x objective. I see this a lot in my work. Since the working distance of the 40x is so close to the slide, what often times will happen is that it will accidentally get dragged through the oil while rotating to lower magnifications. The 100x is sealed to protect it against the oil, but the 40x is not, and if the oil sits on the objective for any length of time, the objective will act like a wick of a candle and **** the oil up inside of it. At that point it is more economical to buy a new object. The same thing can happen with the 100x, but it takes much longer for it to happen. Basically the seal around the objective tip is just latex chalking, and the oil will break it down over time, thereby causing the same affect as with the 40x.

You can try to clean them. If you are lucky the oil is only on the outside surface of the objective. Try using a que-tip and isophrapoyl alcohol to clean it off.

Jan 14, 2011 | Swift M2251C Monocular Microscope

1 Answer

How do I get motic microscope lens repair? It's an older model-a BA 200. Our lens is a EF Plan 40X objective /0.65 /0.17


You can send the lens to Delta Optical Instruments, Inc.They can determine if your EF Plan 40x objective lens is repairable.The will give you an estimate prior to work or suggest a replacement if it cannot be repaired.email them at:  robin@deltaoi.com

Oct 06, 2010 | Motic DMBA 300 Binocular Microscope

1 Answer

What is the function and the purpose of the parts of the microscope ?


Starting at the bottom of the Compound Microscope scope working up: The base of the scope houses the illumination system and the field lens. It also provides weighted stablility.
The field lens is where the light comes out of the base. This lens focuses the light from the bulb directly into the substage condenser.
The arm of the scope supports the rest of the scope.
Directly above the field condenser is the substage condenser. The substage condenser collects the light and condenses it further into a more concentrated beam of light. With the substage condenser you can control the amount of light and to some degree the defraction of light. This is helpful in adjusting the "contrast" in the image.
Working up, you find the "stage" which is a platform to support the specimen. The stage may or may not have a specimen holder and a set of specimen holder control knobs.
The magnifying lenses are called the objectives. They look like barrels pointing down at the stage. These are usually marked 4x, 10x, 40x, and 100x. These magnifications are further multiplied by the power of the eyepieces which are usually 10x. So, when using the 4x you are actually seeing the specimen at 40x and when you are using the 10x objective you are actually seeing the specimen at 100x and so on. The 4x is used to "scan" the specimen so that you can find the most interesting area and then focus in further on that region. It is very hard to find exactly what you want to focus in on starting with the higher magnifications. Always start with the low magnifications, 4x or 10x.
The objective lenses are mounted on a rotating turret for easy selection.
Moving on up the scope is the head. It is comprised of the eye tube and the eyepiece. If you have two eyepieces your scope is referred to as a "Binocular Compound Microscope". If you have only one eyepiece, your scope is a "Monocular Compound Microscope".

Sep 27, 2010 | C And A Scientific IExplore Scope

2 Answers

Problems with viewing under low magnification (20x) only red stain


hello..

For the red colour partical your illumination angle should be 0 degree.
As per you exepirience you are getting red at low magnification and not getting at high magnification. at the higer magnification the light deviation you can apriciate more then lower.

try to adjust your light source focusing lenc at higher magnification so at the lower it will be more better,

for the further problem you can contect me any time,

regards.

INDRAVIJAY

Oct 11, 2007 | Tasco 750 Monocular Microscope

6 Answers

Bacteria?


Hi there,

Seing bacteria isn't just about magnification, many
are transparent and need to be stained. Even at X1000, you will see little detail, but can make approximations of shape etc. Here is a good starting point for staining bacteria.
With a toothpick scrape a little plaque from your teeth (size of a pinhead is plenty). Put this in the centre of a slide with 1 drop of water and mix thoroughly. Allow this to dry then pass the sample through a flame three or four times (hot, but not hot enough to burn fingers) Stain for five mins using either Methylene Blue or Eosin. If you don’t have these, Blue or Red fountain pen ink will do for starters. Rinse off excess stain with very slow running water. Blot dry and observe at X400. If you have an oil immersion lens, you must use a cover slip and mount your specimen in balsam first.
If I can be of any further help, please don’t hesitate to ask.
Cheers…..Dave

Aug 01, 2007 | National Optical 131 Monocular Microscope

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