I have a Nikon Optiphot-2 bright field and phase microscope. I have been searching for a compatible 2x objective lens that works with the Optiphot-2. I tried several Nikon 2x lens with 160 tuble length but they do not work. I do not have a manual of the Optipjot-2. If any one knows THE TUBE LENGTH OF THE OPTIPHOT-2 MICROSCOPE, please let me know. Also if ANY ONE KNOW WHICH NIKON 2X OBJECTIVE LENS MODEL IS COMPLATIBLE WITH THE OPTIPHOT-2, PLEASE let me know. Does any one have a copy of the MANUAL for OPTIPHOT-2 microscope? I would like to have a duplicate copy of such a manual and I will pay for the cost of duplication and mailing service. Thank you very much for your assistance.
The Nikon Optiphot 2 tube length is 160 mm DIN finite. It is a 1990 vintage microscope. Any objective lens with the standard RMS thread, -160 engraved on it will work. It will not work with other brnads with the infinity symbol engraved on it or infinity tube length Nikon 200 mm, CFI60 objectives for 2000 vintage to present microscopes. - Lawsen Lew
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Email nikoninstruments(at)nikon(dot)net with some pictures of your microscope and I'll see if we can ID it. Make sure the pics are bright enough and in focus and at a wide enough angle to get a good look at it. Nikon Instruments Microscopes and Imaging Systems
Open the field and the condenser diaphragms all the way. bring your object into focus then insert the telescope eyepiece which lets you see both phase rings. Bring both the phase rings into focus. Then move the phase ring of the condenser's side until it is lined with the phase ring of the objective and no light gets passed up. Inspect your object now with your regular eyepieces. That is it. Have fun.
In the Optiphot there are two small grub screws under the left hand side of the condenser column. Once loosened the metal housing of the pinion can by pulled or pushed out and the new one inserted. You may need a small mirror to see the screws due to their hidden position. You can then replace the pinion in which the black nylon gear will have broken
Compound microscopes can be
found in most biology and science classrooms. They are electrically
operated and use light to enhance the image of a cell. They will have
multiple lenses for viewing.
Dissecting microscopes are also
known as stereo microscopes. They have low magnification and are also
light powered. These microscopes can view objects larger than what a
compound microscope is able to handle, in three dimensions.
A Scanning Electron Microscope
uses electrons instead of light to create an image. These microscopes
produce three-dimensional images with high resolution and magnification.
They also have a larger depth of focus.
Microscopes use electrons instead of light to create an image. The
material prepared must be very thin. The beams of electrons that pass
through it give the viewer high magnification and resolution. These give
There are a few things that could cause a shadow in the field of view.
First if the condenser is not centered, this can create a crescent shaped shadow and rob your image of light. Center the condenser and raise it to within about a 16th of an inch from the top of the stage.
Second, the slider on the head which diverts light from the eyepieces to the camera must be fully engaged to the "camera" position
Third, make sure that the diaphragm on the condenser is not closed too far. There is an adjustment ring for this. Set it to 70-80% of the numerical aperture of the objective you are using.
Fourth: if there is a "swing out" condenser lens you will need it completely in or out depending on the objective you are using. for 2x-5x you need the swing out condenser lens completely out of the light path. For objectives 10x and up you need the swing out condenser lens completely in and centered in the light path.
If these things are properly adjusted you should not have a shadow in the field of view. If you still have a problem, let me know.
A 2X adapter is an item attached between your lens and camera body. It's AF (which is good) and N (most likely a Nikon). They make what ever zoom lens 2X the focal length. So a 135mm becomes a 270mm. So you get a bigger tele lens. They add extra glass, so the image is not as good without it, they eat up an F/stop. So outdoors in bright sunlight, no problem. It's shady and you have ASA 200 and you are going to have a slow shutter speed that can cause blur. Plus the viewfinder gets darker due to the extra glass. So in dim light, it may not AF and be hard to manual focus.
This might be sign of trouble, because the Nylon gears inside might be worn out. I did not noticed a tension ring on Optiphot 1 or 2. The Nikon S and Metaphot from 1960s to 1976 have Nylon gears with tension ring adjustments.
Let x = the short side. Then the long side is the short side plus 6 meters now we have 2 sides that are x meters long, add those together gives us 2x for the length of both short sides
The length of the long side is x + 6 for one side and x + 6 for the other side. Added together we have x + 6 + x + 6 = 2x + 12
Adding the 4 sides together we get 2x ( the 2 short sides) + 2x + 12 (the long sides) we get 4x + 12 = the perimeter or 628. 4x + 12 = 628. Subtract 12 from both sides of the equation leaves 4x = 616. divide both sides by 4 leaves x = 154 So the width is 154 meters and the length is 160 meters.
You should start by changing the bulb, even though it looks ok chances are that it's no longer working. Do the same with the fuse if the new bulb doesn't work.
Test that power is reaching the socket with a voltmeter or a multimeter so ensure the wiring is ok with the new fuse. If no power is reaching the bulb socket, then you will need to check the wiring every step of the way.