If the thermostat came with a new rubber o ring that fits around it, then no the plastic housing shouldnt need to be sealed, but if there is no rubber o ring yes seal it or it will leak... as far as distilled water, no just reg tap water is fine...remember to put anti-sieze on your bolts before you put them back in.. or they may not come out if you need to replace the thermostat in the future... hope this helps
Please see my tip at http://www.fixya.com/cars/r5929960-s_thermostat .
Specific instructions for your car are as follows. Let me know if you get stuck or have more questions. 5.3L Engine
Removal & Installation
4.2L Engine
To install:
NOTE
The thermostat is not serviceable separately. The water pump inlet and thermostat must be replaced as an assembly.
To install:
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