Vacuum lines will not cause a specific cyl misfire. You may have a bad coil pack, or bad injector. You will need to make sure you have spark at the specific cyl listed for the misfire, and can test to see if you have injector pulse using a noid lite. Also, and good scan tool can show you live data, and allow you to watch the components operation, and give you a good starting point to determine the miss.
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