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Maybe a problem in the fuel pump circuit. Check fuel pressure with engine off, and key turned to on, not start. If no pressure, diagnose the pump circuit at the fuel pump relay. One terminal for the relay would be the power feed for the pump. The engine computer signals the relay on for 2 seconds with key on to prime the system, also signals during engine cranking.
What is the fuel pressure? these trucks need a certain amount of pressure to start, if low just 3psi it will not start, if you are sure the pump is good then you may bad spider injector. check fuel pressure first and also try spraying starting fluid into throttle body and see if truck starts. the pump needs 60 -66 psi to start the truck.
what is the fuel pressure with key on and engine off? since you mentioned spider injector assembly it has to be a vortec engine that requires 55psi to open the poppet valve in the injectors just to start.
that indicates you have a fuel supply problem. a plugged fuel filter, or a fuel pump thats loosing its pressure. usually when you put starting fluid in throttle body. and it starts. thats a fuel problem.
When the fuel pump was replaced ,did the pressure and return lines get put back in the right tubes?What do you mean by a new "injector spider" system,I've never heard the term.Have you checked for bent fuel lines?Where are you doing your pressure tests from?A little more info would be most helpful,thanks.