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The fault code 53 is an ECM IMMOBILIZED ERROR. The immobilizer anti-theft system thinks the key is invalid. If it detects an error, it will; Disable the fuel injector circuit--- Disable the fuel pump circuit---Disable the ignition coil.
Code 53 will set when: 1- The Eectronic Control Module (ECM) does not receive the signal from the Immobilizer Control Unit (ICU) within 0.9 seconds when vehicle is stationary, or 1.8 seconds when vehicle is moving. 2- The ICU sends the ECM an incorrect release message more than 5 times. 3- The above conditions continue until the ignition is switched off.
Just note that even with all the timing marks aligned you can still have the ignition timing 180 degrees incorrect. I.e the intake, exhaust and crankshaft can all be in time but the engine will miss-fire back through the intake due to incorrect IGNITION timing.