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Your Navigation disc usually is in the CD player. Most require the First Disc to be the Navigation disc. Now if this disc was removed or if someone changed the position of #1 disc and inserted something else, then that is why you have your message.
It is also possible that removing the Battery from the car may cause it to lose the programming in the Navigation. It may simply need the Navigation disc to be reread.
Check for a scratched or dirty Navi DVD disc. Clean or have the scratches polish out at your local game store. Video game stores usually have a disc repair machine that polishes out scratches.
Note: In some cases the Navi unit can cause rotational scratches on the DVD. If this is the case than the Navi unit will have to be replaced.
If your using copies then some dvds wont read them even if the old one did, sometimes its a gamble if it reads or not, if your using a master then i would have them check it out, it may be bad or need programing, it funny because sometimes it will play a copy and sometimes not, thats what i've run into. hope this helps.
If you've purchased a new navigation disc and it won't read it, you will probably need to get a firmware update for your navigation unit.
If it won't read an old disc, check the disc for smudge marks or scratches. Clean it and retry. If it still don't work you can try a cleaner that you can get at Radio Shack or similar store.
Hope this helps
most of the time the disc is bad but when the disc goes bad its the navi unit that causes that to go bad so if you replace just disc will need another one soon after recommend replacing unit or ship to repair facilty for repairs
NAV unit uses a DVD disc. If its a copy, it may or may not work. I suggest you remove the NAV disc, unplug the NAV unit from power for 4-5 minutes, reconnect the NAV and try inserting the disc again to see if it reads. If it still does not read, another NAV disc needs to be inserted to verify its not a "bad" NAV DVD disc issue. If it still cannot read the second disc, the NAV unit is bad. The handbook feature needs the disc inserted in the slot 1 of the CD changer unit.
I hate to be the bearer of bad news... that error code means that you have a CD that's bad in the 6 disc changer.... If you're handy... you can take your radio out and manually remove the cd's and make sure that the 6 trays are aligned properly and not stuck together. (known issue) However, Lexus/Toyota does NOT repair ANY of their electrical components. They want you to pay for a NEW one at the tune of 800.00 PLUS installation... and they send the old one back to Japan and remove the CD's and mail them to you. (at no extra cost... as the service rep was telling me.. as to benefit feature me on the sale of the replacement unit.) Next be ready for the "Disc Read Error, Check Disc" on your navigation... lol.. it's a 2.10 motor that moves the lens that reads the DVD navigation CD.. and once again .. they don't replace or repair... that's a 4500.00 item plus installation.. GOTTA LOVE YOUR EXPENSIVE CAR NO? ;)